Catalogue


Non-viral gene therapy [electronic resource] : gene design and delivery /
K. Taira, K. Kataoka, T. Niidome, eds.
imprint
Tokyo ; New York : Springer, c2005.
description
xii, 487 p. : ill., ports.
ISBN
4431251227 (alk. paper), 9784431251224 (alk. paper)
format(s)
Book
More Details
imprint
Tokyo ; New York : Springer, c2005.
isbn
4431251227 (alk. paper)
9784431251224 (alk. paper)
restrictions
Licensed for access by U. of T. users.
catalogue key
8009550
 
Includes bibliographical references and index.
A Look Inside
Summaries
Main Description
The development of gene medicine based on the concept of molecular therapy has opened new medical horizons. Among the many techniques in gene medicine, gene design and delivery are especially significant in clinical applications. This book presents state-of-the-art information on non-viral gene-delivery techniques, covering a broad spectrum of disciplines that include chemistry, molecular biology, cell biology, and pharmacokinetics. Major sections introduce molecules for gene delivery and their properties; technologies of controlled gene delivery in vitro and in vivo; therapeutic genes and the status of clinical applications; and the design of genes based on current RNA technology, with revolutionary developments in the world of RNAi shown to be key factors in gene medicine. This groundbreaking work is an invaluable resource for researchers and engineers in genetic engineering, molecular medicine, biochemical engineering, and biotechnology.
Table of Contents
Recent progress in nonviral gene deliveryp. 3
Physical chemistry of DNA-carrier complexp. 11
Liposomesp. 19
Polymeric gene carriersp. 35
Development of supramolecular nanocarrier for gene delivery based on cationic block copolymersp. 51
Chitosanp. 63
Dendrimers as DNA carriersp. 75
Use of synthetic peptides for non-viral gene deliveryp. 87
An oligonucleotide carrier based on [beta]-1,3-glucansp. 103
Biological and chemical hybrid vectorsp. 118
Pharmacokinetics of gene delivery in cellsp. 135
Active DNA release from complexesp. 155
Controlled release of DNA using thermoresponsive polymersp. 165
Active transport of exogenous gene into the nucleusp. 176
Controlled intracellular localization of oligonucleotides by chemical conjugationp. 187
Functional nucleotide sequences capable of promoting non-viral genetic transferp. 198
Shielding of cationic charge of the DNA complex to avoid nonspecific interactions for in vivo gene deliveryp. 211
In vivo gene transfer by ligand modified gene carriersp. 226
Optimizing polyplexes into systemic viruses for tumor-targeted gene therapyp. 237
Gene transfer and target diseasesp. 246
Clinical trials using non-viral gene delivery systemsp. 261
Evaluation of size and zeta-potential of DNA/carrier complexesp. 293
Observation of DNA/carrier complex under fluorescence microscopyp. 300
Synthesis of chemically modified chitosan and its gene transfection efficiencyp. 307
Newly designed DNA fragments for gene correctonp. 315
Evaluation of gene expression in vivo after intraveous and intraportal administration of lipoplexesp. 323
Evaluation of southern blot hybridization of DNA administrated with a gene carrier to organsp. 331
Evaluation of immune response after administration of plasmid DNA-nonviral vector complexesp. 339
Gateway RNAip. 348
Design of intracellulary active ribozymes and siRNAp. 363
RNAi-based inhibition specific for mutant alleles in autosomal dominant diseases : sequence-dependent and -independent discrimination of mutant and wild-type alleles by siRNAp. 398
In vivo RNA interference : another tool in the box?p. 405
Suppression of gene expression via chromatin remodeling and the siRNA-Induced silencing of transcriptionp. 423
Intracellular delivery of nucleic acids : differences between transfection and siFection reflect differences between DNA and RNA, and between oligodeoxynucleotides and oligonucleotidesp. 441
In vivo antitumour activity of a new cationic liposome siRNA complexp. 456
Enhancing RNAi with synthetic RNA duplexesp. 465
Table of Contents provided by Blackwell. All Rights Reserved.

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