Catalogue


Gene discovery for disease models [electronic resource] /
edited by Weikuan Gu and Yongjun Wang.
imprint
Hoboken, N.J. : Wiley, c2011.
description
xiii, 537 p., [16] p. of plates : ill. (some col.) ; 25 cm.
ISBN
9780470499467 (cloth)
format(s)
Book
More Details
added author
imprint
Hoboken, N.J. : Wiley, c2011.
isbn
9780470499467 (cloth)
restrictions
Licensed for access by U. of T. users.
contents note
Introduction : gene discovery-from positional cloning to genomic cloning -- High throughput gene expression analysis and the identification of expression QTLs -- DNA methylation in the pathogenesis of autoimmunity -- Ccell-based analysis with microfluidic chip -- Missing dimension : protein turnover rate measurement in gene discovery -- Bioinformatics tools for the prediction of gene function -- Determination of genomic locations of targeted genetic loci -- Mutation discovery using high throughput mutation screening technology -- Candidate screening through gene expression profile -- Candidate screening through high-density SNP array -- Gene discovery through direct genome sequencing -- Candidate screening through bioinformatics tools -- Using an integrative strategy to identify mutations -- Determination of the function of a mutant in a gene -- Confirmation of a mutation by multiple molecular approaches -- Confirmation of a mutation by microRNA -- Confirmation of function of a gene by translational approaches -- Confirmation of single nucleotide mutations -- Initial identification and confirmation of a QTL gene -- Gene discovery of crop diseases in the post genome era -- Impact of whole genome genetic element analysis on gene discovery of disease models -- Impact of whole genome protein analysis on gene discovery of disease models.
catalogue key
7929247
 
Includes bibliographical references and index.
A Look Inside
About the Author
Author Affiliation
Weikuan Gu received his PhD from Cornell University in 1994 and joined the University of Tennessee Health Science Center as an assistant professor in 2002. Dr. Gu's lab has developed an integrated strategy for the positional cloning of genes, a strategy which has been successfully applied to clone several genes from spontaneous mouse mutations. Yongjun Wang received his MD from Hebei Medical College in 1982 and his MBA from Peking University in 2004. Dr. Wang joined Beijing Tiantan Hospital in 2000 and now serves as the vice president of the hospital.
Reviews
This item was reviewed in:
Reference & Research Book News, June 2011
To find out how to look for other reviews, please see our guides to finding book reviews in the Sciences or Social Sciences and Humanities.
Summaries
Back Cover Copy
New concepts in gene discovery in the post-genome era The completion of human and other genome sequencing, along with other new technologies, such as mutation analysis and microarray, has dramatically accelerated progress in the positional cloning of genes from mutated models. Gene Discovery for Disease Models provides readers with a comprehensive understanding of the new concepts and protocols implemented in gene discovery in the present post-genome era. Backed by sound scientific findings, this informative guide not only provides a systematic introduction to the available resources and technologies for gene discovery but, most importantly, teaches readers how to use all the available tools and data to find new mutated genes. Its comprehensive coverage: Provides a detailed description of positional cloning and genomic cloning Identifies genes of human disease and animal models Suggests new paradigms for mutation discovery in the post-genome era Describes new concepts in gene discovery in the post-genome era and the use of streamlined protocols to identify genes of interest Gene Discovery for Disease Models helps researchers to not only understand the current concepts and technologies, but also learn how to take advantage of these new resources and technologies in the future and adapt to emerging new discoveries in the genetic sciences. This book can be used as a handbook for gene cloning and discovery, as well as a reference book for teachers and students in the fields of genetics and biology.
Main Description
The completion of human and other genome sequencing, along with other new technologies, such as mutation analysis and microarray, has dramatically accelerated the progress in positional cloning of genes from mutated models. Gene Discovery for Disease Models provides readers with new paradigms on the mutation discovery in the post-genome era with detailed description of positional cloning and genomic cloning with identification of genes found in human disease and animal models. Backed by sound scientific findings, this informational guide advances the promising possibilities of genetic research.
Main Description
This book provides readers with new paradigms on the mutation discovery in the post-genome era. The completion of human and other genome sequencing, along with other new technologies, such as mutation analysis and microarray, has dramatically accelerated the progress in positional cloning of genes from mutated models. In 2002, the Mouse Genome Sequencing Consortium stated that "The availability of an annotated mouse genome sequence now provides the most efficient tool yet in the gene hunter's toolkit. One can move directly from genetic mapping to identification of candidate genes, and the experimental process is reduced to PCR amplification and sequencing of exons and other conserved elements in the candidate interval. With this streamlined protocol, it is anticipated that many decades-old mouse mutants will be understood precisely at the DNA level in the near future." The implication of such a statement should be similar to the identification of mutated genes from human diseases and animal models, when genome sequencing is completed for them. More than five years have passed, but genes in many human diseases and animal models have not yet been identified. In some cases, the identification of the mutated genes has been a bottleneck, because the genetic mechanism holds the key to understand the basis of the diseases. However, an integrative strategy, which is a combination of genetic mapping, genome resources, bioinformatics tools, and high throughput technologies, has been developed and tested. The classic paradigm of positional cloning has evolved with completely new concepts of genomic cloning and protocols. This book describes new concepts of gene discovery in the post-genome era and the use of streamlined protocols to identify genes of interest. This book helps identify not only large insertions/deletions but also single nucleotide mutations or polymorphisms that regulate quantitative trait loci (QTL).
Table of Contents
Prefacep. vii
Acknowledgmentsp. ix
Contributorsp. xi
Gene Discovery: From Positional Cloning to Genomic Cloningp. 1
High-Throughput Gene Expression Analysis and the Identification of Expression QTLsp. 11
DNA Methylation in the Pathogenesis of Autoimmunityp. 31
Cell-Based Analysis with Microfluidic Chipp. 59
Missing Dimension: Protein Turnover Rate Measurement in Gene Discoveryp. 83
Bioinformatics Tools for Gene Function Predictionp. 93
Determination of Genomic Locations of Target Genetic Locip. 111
Mutation Discovery Using High-Throughput Mutation Screening Technologyp. 139
Candidate Screening through Gene Expression Profilep. 165
Candidate Screening through High-Density SNP Arrayp. 195
Gene Discovery by Direct Genome Sequencingp. 215
Candidate Screening through Bioinformatics Toolsp. 235
Using an Integrative Strategy to Identify Mutationsp. 261
Determination of the Function of a Mutationp. 279
Confirmation of a Mutation by Multiple Molecular Approachesp. 303
Confirmation of a Mutation by MicroRNAp. 343
Confirmation of Gene Function Using Translational Approachesp. 371
Confirmation of Single Nucleotide Mutationsp. 391
Initial Identification and Confirmation of a QTL Genep. 403
Gene Discovery of Crop Disease in the Postgenome Erap. 425
Impact of Genomewide Structural Variation on Gene Discoveryp. 443
Impact of Whole Genome Protein Analysis on Gene Discovery of Disease Modelsp. 471
Indexp. 531
Table of Contents provided by Ingram. All Rights Reserved.

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