Catalogue


DNA vaccines [electronic resource] : methods and protocols /
edited by Douglas B. Lowrie and Robert G. Whalen.
imprint
Totowa, N.J. : Humana Press, c2000.
description
xix, 529 p. : ill. ; 24 cm.
ISBN
0896035808 (alk. paper), 9780896035805 (alk. paper)
format(s)
Book
Subjects
genre index term
More Details
imprint
Totowa, N.J. : Humana Press, c2000.
isbn
0896035808 (alk. paper)
9780896035805 (alk. paper)
restrictions
Licensed for access by U. of T. users.
catalogue key
7894865
 
Includes bibliographical references and index.
A Look Inside
Reviews
Review Quotes
"...does an excellent job of describing the current methodologies in their development, what clinical benefit can be expected, and what is known of their mode of action...has thoroughly covered the current state of the art." -Antiviral Research "The present book contains 41 chapters by experts in the field not only describing the state-of-the-art thinking on each topic, but, more importantly, providing a step-by-step recipe for constructing the cost effective plasmids and measuring host immune responses, both humoral and cellular, as well as all the stops in between including delivery, etc....an excellent roadmap as to how to proceed...This comprehensive collection of chapters is the first of its kind in the rapidly moving field to provide an excellent update of all of the recent advances and, more importantly, the actual technical approaches to developing vaccines. This is a book that should be in everyone's library who is interested in pursing studies employing DNA immunization and for graduate students interested in an up-to-date understanding of DNA vaccination." -FEBS Letters "Its 41 chapters provide a valuable introduction, a series of working protocols, and a set of heavily referenced reviews of state-of-the-art methods in the production, use, and analysis of DNA vaccines. The various chapters are especially fruitful in their presentation of detailed techniques for making buffers and complex biological reagents." -Modern Drug Discovery "Tecular Biology series, consists of 41 well written and referenced chapters dealing with certain aspects of DNA vaccination. The subjects addressed range from large scale plasmid isolation to DNA vaccination and autoimmunity, and illuminate the explosive growth this field has undergone in recent years. A substantial amount of the text concentrates on the practical aspects of processing DNA, quality control and immunological responses....In short, this book is a valuable addition to the library of all people actively involved in the field of DNA vaccination. Researchers will find the detailed protocols and innumerable reference valuable. I would recommend this book to any researcher in this field, if only to finally have a book with well organized and referenced protocols instead of a pile of coffee stained photocopies." -Today's Life Science "The scope of the text is wide and covers virtually all aspects of the subject from the initial plasmid preparation and industrial scale up to the regulatory issues that will probably need to be addressed if such vaccines reach licensing stage." - The Journal of Medical Microbiology "Work on the development of DNA vaccines continues aspace for a huge range of potential applications and this book will be invaluable for any laboratory involved in research in this field." -Microbiology Today
"...does an excellent job of describing the current methodologies in their development, what clinical benefit can be expected, and what is known of their mode of action...has thoroughly covered the current state of the art." -Antiviral Research"The present book contains 41 chapters by experts in the field not only describing the state-of-the-art thinking on each topic, but, more importantly, providing a step-by-step recipe for constructing the cost effective plasmids and measuring host immune responses, both humoral and cellular, as well as all the stops in between including delivery, etc....an excellent roadmap as to how to proceed...This comprehensive collection of chapters is the first of its kind in the rapidly moving field to provide an excellent update of all of the recent advances and, more importantly, the actual technical approaches to developing vaccines. This is a book that should be in everyone's library who is interested in pursing studies employing DNA immunization and for graduate students interested in an up-to-date understanding of DNA vaccination." -FEBS Letters"Its 41 chapters provide a valuable introduction, a series of working protocols, and a set of heavily referenced reviews of state-of-the-art methods in the production, use, and analysis of DNA vaccines. The various chapters are especially fruitful in their presentation of detailed techniques for making buffers and complex biological reagents." -Modern Drug Discovery"Tecular Biology series, consists of 41 well written and referenced chapters dealing with certain aspects of DNA vaccination. The subjects addressed range from large scale plasmid isolation to DNA vaccination and autoimmunity, and illuminate the explosive growth this field has undergone in recent years. A substantial amount of the text concentrates on the practical aspects of processing DNA, quality control and immunological responses....In short, this book is a valuable addition to the library of all people actively involved in the field of DNA vaccination. Researchers will find the detailed protocols and innumerable reference valuable. I would recommend this book to any researcher in this field, if only to finally have a book with well organized and referenced protocols instead of a pile of coffee stained photocopies." -Today's Life Science"The scope of the text is wide and covers virtually all aspects of the subject from the initial plasmid preparation and industrial scale up to the regulatory issues that will probably need to be addressed if such vaccines reach licensing stage." - The Journal of Medical Microbiology"Work on the development of DNA vaccines continues aspace for a huge range of potential applications and this book will be invaluable for any laboratory involved in research in this field." -Microbiology Today
This item was reviewed in:
SciTech Book News, December 1999
To find out how to look for other reviews, please see our guides to finding book reviews in the Sciences or Social Sciences and Humanities.
Summaries
Back Cover Copy
In DNA Vaccines: Methods and Protocols, state-of-the-art review articles by leading experts summarize how to develop and employ the highly promising new DNA vaccines, what clinical results can be expected from their use, and what is known about how they work. Key topics range from vaccine design and construction to preparation and delivery methods, including the use of classical adjuvants, "genetic adjuvants," and the immunostimulatory properties of DNA and selected oligonucleotide sequences. Several contributors provide strategic ideas on antigen engineering and describe the particularly novel applications of DNA vaccine methodology that have recently emerged. In addition, there is a discussion of dendritic cells and antigen-presenting cells, the understanding of whose actions holds great promise for modulating the immune response, and thus for treating disease. Cutting-edge and comprehensive, DNA Vaccines: Methods and Protocols provides a broad panorama of the methods and thinking from which the vaccines of tomorrow will evolve, and so constitutes an invaluable sourcebook for both experts developing new applications and newcomers who want to gain mastery of the techniques and problems involved.
Main Description
State-of-the-art review articles by leading experts summarize how to develop and employ the highly promising new DNA vaccines, what clinical results can be expected from their use, and what is known about how they work. Key topics range from vaccine design and construction to preparation and delivery methods, including the use of classical adjuvants, "genetic adjuvants," and the immunostimulatory properties of DNA and selected oligonucleotide sequences. Several contributors provide strategic ideas on antigen engineering and describe the novel applications of DNA vaccine methodology that have recently emerged. Cutting-edge and comprehensive, DNA Vaccines: Methods and Protocols provides a snapshot of the methods and thinking from which the vaccines of tomorrow will evolve.
Unpaid Annotation
State-of-the-art methods by leading experts summarize how to develop and employ the highly promising new DNA vaccines, what can be expected from their clinical development, and what is known about how they work. The articles include coverage of cutting-edge topics such as immunostimulatory sequences, cytokines, and other adjuvants, as well as practical advice for general and specific applications. The book will serve as an invaluable sourcebook for newcomers who want to get a feel for the variety of techniques and problems involved and for experts who want to develop new applications.
Table of Contents
Prefacep. v
Contributorsp. xiii
Purification of Supercoiled Plasmidp. 1
Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelinesp. 11
Development and Characterization of Lyophilized DNA Vaccine Formulationsp. 23
Repeated Use of Qiagen Columns in Large-Scale Preparation of Plasmid DNAp. 35
The Immunology of DNA Vaccinesp. 37
Methodology Used in DNA-Based Prophylactic and Therapeutic Immunization Against Hepatitis B Virus in Chimpanzeesp. 65
Intramuscular and Intradermal Injection of DNA Vaccines in Mice and Primatesp. 71
Veterinary DNA Vaccinesp. 79
DNA-Based Immunization of Neonatal Micep. 95
Intramuscular Injection of DNA Vaccines in Fishp. 99
Development of DNA Vaccines for Salmonid Fishp. 105
CTL Analysis for Tumor Vaccinesp. 123
The Use of Bone Marrow-Chimeric Mice in Determining the MHC Restriction of Epitope-Specific Cytotoxic T Lymphocytesp. 133
Immunostimulatory DNA Sequences: An Overviewp. 145
Immunostimulatory CpG Motifs and DNA Vaccinesp. 169
In Vitro Assay of Immunostimulatory Activities of Plasmid Vectorsp. 173
Adjuvants for Plasmid DNA Vaccinesp. 185
Cytokine and Costimulatory Factor-Encoding Plasmids as Adjuvants for DNA Vaccinationp. 197
Optimization of DNA Vaccines Through the Use of Molecular Adjuvantsp. 205
Cytokine Fusion Constructs as DNA Vaccines Against Tumorsp. 221
The Use of Conventional Immunologic Adjuvants in DNA Vaccine Preparationsp. 241
Genetic Adjuvantsp. 251
DNA Immunization in Combination with the Immunostimulant Monophosphoryl Lipid Ap. 261
Controlled Plasmid Delivery and Gene Expression: Applications for Nucleic Acid-Based Vaccinesp. 267
Mucosal Immunization with DNA Vaccinesp. 287
Preparations for Particle-Mediated Gene Transfer Using the Accell Gene Gunp. 297
Entrapment of Plasmid DNA Vaccines into Liposomes by Dehydration/Rehydrationp. 305
DNA-Based Vaccination with Polycistronic Expression Plasmidsp. 313
A Nonviral Cytoplasmic T7 Autogene System and Its Applications in DNA Vaccinationp. 323
Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRESp. 335
Genetic Subunit Vaccines: A Novel Approach for Genetic Immunizationp. 345
Antigen Engineering in DNA Immunizationp. 355
Genetic Vaccination Targeting T-Cell Receptorsp. 375
Immunity to T-Cell Receptor: Suppressive Vaccination with DNA Encoding a Variable Region Gene of the T-Cell Receptorp. 397
DNA Fusion Vaccines Against B-Cell Tumorsp. 405
DNA-Based Vaccination Primes Tumor-Rejecting T-Cell Responsesp. 425
Development of a Female Contraceptive Vaccine Through DNA Inoculation of Human Chorionic Gonadotropin Beta Subunit (hCG[beta])p. 439
Genetic Immunization for Allergy Immunotherapyp. 451
Preclinical Safety of DNA Vaccines: A Method to Analyze the Distribution of Plasmid DNA in Animal Modelsp. 473
DNA Vaccination: Tolerance and Autoimmunityp. 489
Assuring the Quality, Safety, and Efficacy of DNA Vaccinesp. 499
Indexp. 509
Table of Contents provided by Syndetics. All Rights Reserved.

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