Catalogue


Accessing uncultivated microorganisms : from the environment to organisms and genomes and back /
edited by Karsten Zengler.
imprint
Washington, D.C. : ASM Press, c2008.
description
xii, 308 p. : ill. (some col.) ; 26 ; cm.
ISBN
1555814069 (hardcover : alk. paper), 9781555814069 (hardcover : alk. paper)
format(s)
Book
Holdings
More Details
added author
imprint
Washington, D.C. : ASM Press, c2008.
isbn
1555814069 (hardcover : alk. paper)
9781555814069 (hardcover : alk. paper)
contents note
Does cultivation still matter? / Karsten Zengler -- The human intestinal microbiota and its impact on human health / Mirjana Rajili-Stojanovi, Willem M. de Vos, and Erwin G. Zoetendal -- The uncountables / William T. Sloan, Christopher Quince, and Thomas P. Curtis -- The missing fungi : new insights from culture-independent molecular studies of soil / S.K. Schmidt ... [et al.] -- The diversity of free-living protists seen and unseen, cultured and uncultured / David A. Caron and Rebecca J. Gast -- Microbial biogeography : patterns in microbial diversity across space and time / Noah Fierer -- The least common denominator : species or operational taxonomic units? / Ramon Rosselló-Mora and Arantxa López-López --
Measuring diversity / Jed A. Fuhrman -- Metagenomics as a tool to study biodiversity / Karen E. Nelson -- New cultivation strategies for terrestrial microorganisms / Peter H. Janssen -- Cultivation of marine symbiotic microorganisms / Todd A. Ciche -- Methods to study consortia and mixed cultures / Boran Kartal and Marc Strous -- Microbial cell individuality / Simon V. Avery -- Nanomechanical methods to study single cells / Ramya Desikan ... [et al.] -- Single cell genomics / Martin Keller, Christopher W. Schadt, and Anthony V. Palumbo -- How many genes does a cell need? / Hamilton O. Smith ... [et al.].
catalogue key
6664036
 
Includes bibliographical references and index.
A Look Inside
Full Text Reviews
Appeared in Choice on 2008-12-01:
Microbiologists have long known that the microbes they are able to cultivate represent only a small fraction of those found in nature. However, due to the revolution in genetic techniques, scientists now have tools to better understand the world of the uncultivatables. This volume, edited by Zengler (Univ. of California, San Diego), summarizes some of the recent developments in metagenomics and metaproteomics, advances in cultivation, and the analysis of single microbial cells. Some of the 16 chapters relate to specific environments (e.g., human gut) or to specific types of organisms (fungi and Protista). Others address the use of direct sequencing of DNA and RNA from the environment. There is also a section on newer cultivation techniques, including those that allow the cultivation of symbiotic microbes and mixed cultures. Four chapters discuss techniques for examining the genetic and metabolic characteristics of single cells. The last chapter focuses on the question of the minimum number of genes required by a cell. This volume would be useful only for a researcher in the field of microbial ecology who is specifically dealing with diversity, abundance, and taxonomy of uncultivated microbes. Each chapter is a rich source of references, ranging from the late 1880s to 2007. Summing Up: Recommended. Researchers and faculty. R. Seelke University of Wisconsin--Superior
Reviews
This item was reviewed in:
Choice, December 2008
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Summaries
Bowker Data Service Summary
Providing a comprehensive overview and discussing developments in the field, this book details various innovative methods used in microbial ecology and environmental microbiology. It also includes all aspects of microbial diversity from bacteria and fungi to protists.
Long Description
Microbes are extremely diverse, yet only a tiny fraction of organisms present in any given environment can be cultivated in the laboratory. Evaluating microbial diversity and identifying individual organisms responsible for specific processes in the environment are fundamental and challenging tasks in microbial ecology and applied microbiology. This comprehensive, judicious, and useful volume provides a complete overview of methods and approaches to studying microbial diversity. While emphasizing new innovative methods, the book also addresses established methods and provides hands on advice on how to isolate, cultivate, and study organisms.Depending on the scope of a study, there are many different ways to assess the diversity of bacteria, fungi, and protists. This volume examines the variety of these approaches-and their advantages and drawbacks. From direct measurements of physical, chemical, and biological aspects of the environment to a wide array of molecular tools such as metagenomic and metaproteomic approaches, this volume thoroughly covers the range of assessment possibilities. It also details innovative methods such as the use of genetic information from environmental samples, enrichment cultures, and single cells. Additionally, the book offers practical, essential advice on how to put these methods into practice both within and outside of the laboratory environment.
Main Description
Provides a comprehensive overview and discusses the latest developments in the field Details various innovative methods for assessment of microbes both within and outside of the laboratory Reviews many different methods used in microbial ecology and environmental microbiology Presents chapters authored by world-renowned experts in the field Highlights various aspects of assessing microorganism from the environment Includes all aspects of microbial diversity from bacteria and fungi to protists Presents a crucial "how to" guide for isolating organisms from different environments
Main Description
Microbes are extremely diverse, yet only a tiny fraction of organisms present in any given environment can be cultivated in the laboratory. Evaluating microbial diversity and identifying individual organisms responsible for specific processes in the environment are fundamental and challenging tasks in microbial ecology and applied microbiology. This comprehensvie and useful volume provides a complete overview of methods and approaches to studying microbial diversity. While emphasizing new innovative methods, the book also addresses established methods and provides "hands on" advice on how to isolate, cultivate, and study organisms.
Table of Contents
Contributorsp. vii
Prefacep. xi
Introductionp. 1
Does Cultivation Still Matter?p. 3
The Human Intestinal Microbiota and Its Impact on Healthp. 11
The Status Quo: Who is Out There? How to Determine Microbial Diversityp. 33
The Uncountablesp. 35
The Missing Fungi: New Insights from Culture-Independent Molecular Studies of Soilp. 55
The Diversity of Free-Living Protists Seen and Unseen, Cultured and Unculturedp. 67
Microbial Biogeography: Patterns in Microbial Diversity across Space and Timep. 95
The Least Common Denominator: Species or Operational Taxonomic Units?p. 117
Measuring Diversityp. 131
Metagenomics as a Tool To Study Biodiversityp. 153
Are Microorganisms Nonculturable or Not Yet Culturable?p. 171
New Cultivation Strategies for Terrestrial Microorganismsp. 173
Cultivation of Marine Symbiotic Microorganismsp. 193
Methods To Study Consortia and Mixed Culturesp. 205
Do We Have to Change Gear? New Cultivation Approaches and New Molecular Approaches Combinedp. 221
Microbial Cell Individualityp. 223
Nanomechanical Methods To Study Single Cellsp. 245
Single-Cell Genomicsp. 267
How Many Genes Does a Cell Need?p. 279
Indexp. 301
Table of Contents provided by Ingram. All Rights Reserved.

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