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Expression and analysis of recombinant ion channels : from structural studies to pharmacological screening /
edited by Jeffrey J. Clare and Derek J. Trezise.
imprint
Weinheim : Wiley-VCH, c2006.
description
xxix, 273 p. : ill. (some col.) ; 25 cm.
ISBN
3527312099 (hbk. : acid free paper), 9783527312092
format(s)
Book
Holdings
Subjects
More Details
imprint
Weinheim : Wiley-VCH, c2006.
isbn
3527312099 (hbk. : acid free paper)
9783527312092
restrictions
Online version licensed for access by U. of T. users.
catalogue key
6064233
 
Includes bibliographical references and index.
A Look Inside
Reviews
This item was reviewed in:
SciTech Book News, June 2006
To find out how to look for other reviews, please see our guides to finding book reviews in the Sciences or Social Sciences and Humanities.
Summaries
Back Cover Copy
Aimed at filling the gap created by recent technical development sin several key areas, this book is directed at both the new and the experienced researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. Covering many of these new techniques for the first time, the book contains contributions from authors who routinely apply these approaches in key academic labs or major pharmaceutical companies. Rather than describing detailed protocols, to which the reader is directed by key references in each chapter, the authors instead provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere. The results is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial researchers alike.
Back Cover Copy
Aimed at filling the gap created by recent technical development sin several key areas, this book is directed at both the new and the experienced researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. Covering many of these new techniques for the first time, the book contains contributions from authors who routinely apply these approaches in key academic labs or major pharmaceutical companies.Rather than describing detailed protocols, to which the reader is directed by key references in each chapter, the authors instead provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere.The results is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial researchers alike.
Bowker Data Service Summary
This work examines the latest techniques for ion channel analysis which have entered the market in the last five years. The contributors share their experiences and provide valuable practical tips.
Main Description
Filling the gap created over the past five years, during which many new techniques have entered the market, this book is directed at both the new and the experienced ion channel researcher wishing to learn more about the considerations and methods for studying recombinant ion channels.These latest developments are covered here for the first time, contributed by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function studies, from basic approaches for manipulating ion channel cDNAs to more specialized but powerful techniques. This is followed by reviews of strategies and methodologies available for expressing channels in mammalian cells and for their analysis by patch-clamp electrophysiology. Chapters 6 to 8 review the latest methodologies for ion channel drug discovery, including high throughput screening using fluorescence and luminescence, as well as automated planar array electrophysiology. The remaining two chapters focus on approaches for determining ion channel crystal structures and on computational approaches to understanding channel mechanisms at atomic resolution.Rather than provide detailed protocols, indicated by references in each chapter, the authors provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere.The result is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial workers alike.
Main Description
Filling the gap created over the past five years, during which many new techniques have entered the market, this book is directed at both the new and the experienced ion channel researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. These latest developments are covered here for the first time, contributed by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function studies, from basic approaches for manipulating ion channel cDNAs to more specialized but powerful techniques. This is followed by reviews of strategies and methodologies available for expressing channels in mammalian cells and for their analysis by patch-clamp electrophysiology. Chapters 6 to 8 review the latest methodologies for ion channel drug discovery, including high throughput screening using fluorescence and luminescence, as well as automated planar array electrophysiology. The remaining two chapters focus on approaches for determining ion channel crystal structures and on computational approaches to understanding channel mechanisms at atomic resolution. Rather than provide detailed protocols, indicated by references in each chapter, the authors provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere. The result is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial workers alike.
Table of Contents
Introduction - Definitions
Nutritional Genomics: Concepts, Tools and Expectations
Nutrigenomics (Nutrient-Gene Interactions)
Nuclear Receptors: An Overview Mechanism of Action and Cancer
Therapeutic Potential of Retinoids Nuclear
Receptors and the Control of Gene Expression by Fatty Acids Cellular Adaptation to Amino Acid Availability
Mechanisms Involved in the Regulation of Gene Expression
Transcriptional Regulation of Hepatic Genes by Insulin and Glucose
PPARs: Lipid Sensors that Regulate Cell Differentiation Processes Advances in Selenoprotein Expression:
Patterns and Individual Variations PPARs in Atherosclerosis Protein
Synthesis and Cancer Mutations in the PPAR Gene Relevant for Diabetes and the Metabolic Syndrome
Regulation of Lipogenic Genes in Obesity
Nutrigenetics (Nutrient-Genotype Interactions)
The Genetics of Type 2 Diabetes Gene Variants and Obesity Gene Polymorphisms
Nutrition, and the Inflammatory Response Gene Variants, Nutritional Parameters, and Hypertension Gene Variants
Nutrition, and Cancer Taste Receptors and their Variants Cancer and Gene Variants in Enzymes Metabolizing Dietary Xenobiotics
Table of Contents provided by Publisher. All Rights Reserved.

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