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Xenopus protocols : cell biology and signal transduction /
edited by X. Johné Liu.
imprint
Totowa, N.J. : Humana Press, c2006.
description
xv, 489 p. : ill. ; 24 cm.
ISBN
1588293629 (alk. paper)
format(s)
Book
Holdings
More Details
added author
imprint
Totowa, N.J. : Humana Press, c2006.
isbn
1588293629 (alk. paper)
catalogue key
5861581
 
Includes bibliographical references.
A Look Inside
Reviews
This item was reviewed in:
SciTech Book News, June 2006
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Summaries
Back Cover Copy
Although Xenopus oocytes are very hardy and robust in terms of experimentation and offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cytoplasm and DNA to the nucleus, they are underutilized as an experimental model. In Xenopus Protocols: Cell Biology and Signal Transduction, hands-on researchers describe standard and cutting-edge techniques for using Xenopus oocytes and oocyte/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance (for example, 50-70 ng catalytic subunit of PKA per oocyte), its striking protein translation capacity (200-400 ng protein/oocyte per day), and its breathtaking possibilities for the assembly of infectious viral particles by single-cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction and cover all the major uses of oocytes/extracts as experimental models. The protocols follow the successful Methods in Molecular Biology'„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Xenopus Protocols: Cell Biology and Signal Transduction serves perfectly to instruct novices in the use of Xenopus oocytes/extracts, as well as seasoned investigators interested in developing novel models and uses.
Main Description
A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models.
Unpaid Annotation
This volume complements the larger body of literature on the subject by focusing on the versatility of frog oocytes and egg extracts in cell biology and signal transduction. All of the unique advantages of using frog oocytes as an experimental model are enthusiastically presented including pioneering work in the assembly of infectious virus particles via co-injection of genomic viral nucleic acids and capsid mRNAs. Xenopus Protocols: Cell Biology and Signal Transduction serves the scientific community by outlining the many possibilities this system has to offer and explores the possibility that Xenopus oocytes will serve prominently as a cell-based model to study functional genomics and proteomics in the post-genomic era.
Table of Contents
Prefacep. v
Contributorsp. xi
Resources for Genetic and Genomic Studies of Xenopusp. 1
The Physiology of the Xenopus laevis Ovaryp. 17
Oocyte Isolation and Enucleationp. 31
Xenopus tropicalis Oocytes: More Than Just a Beautiful Genomep. 43
Oocyte Expression With Injection of Purified T7 RNA Polymerasep. 55
Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microscopyp. 69
Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Researchp. 87
Imaging Ca2+ Signals in Xenopus Oocytesp. 103
Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggsp. 121
Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytesp. 139
Pre-mRNA Splicing in the Nuclei of Xenopus Oocytesp. 149
Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpolesp. 165
Cytoplasmic mRNA Polyadenylation and Translation Assaysp. 183
Xenopus Egg Extracts: A Model System to Study Proprotein Convertasesp. 199
Analysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding Assayp. 213
Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extractsp. 223
Introduction to Nucleocytoplasmic Transport: Molecules and Mechanismsp. 235
Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studiesp. 259
Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopyp. 273
In Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg Extractsp. 289
Use of Intact Xenopus Oocytes in Nucleocytoplasmic Transport Studiesp. 301
Studying the Mechanosensitivity of Voltage-Gated Channels Using Oocyte Patchesp. 315
Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticidesp. 331
Microtransplantation of Neurotransmitter Receptors From Cells to Xenopus Oocyte Membranes: New Procedure for Ion Channel Studiesp. 347
Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extracts in Semi-Intact MDCK Cellsp. 357
Exploring RNA Virus Replication in Xenopus Oocytesp. 367
Study of Apoptosis in Vitro Using the Xenopus Egg Extract Reconstitution Systemp. 379
Studying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and Proteomicsp. 395
Localized Sampling, Electrophoresis, and Biosensor Analysis of Xenopus laevis Cytoplasm for Subcellular Biochemical Assaysp. 413
Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cellsp. 425
Using Xenopus Oocyte Extracts to Study Signal Transductionp. 435
Oocyte Extracts for the Study of Meiotic M-M Transitionp. 445
Methods for Studying Spindle Assembly and Chromosome Condensation in Xenopus Egg Extractsp. 459
Indexp. 475
Table of Contents provided by Publisher. All Rights Reserved.

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