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Separation methods in proteomics /
edited by Gary B. Smejkal , Alexander Lazarev.
imprint
Boca Raton : Taylor & Francis, 2006.
description
510 p.
ISBN
0824726995 (alk. paper), 9780824726997 (alk. paper)
format(s)
Book
Holdings
More Details
imprint
Boca Raton : Taylor & Francis, 2006.
isbn
0824726995 (alk. paper)
9780824726997 (alk. paper)
contents note
"Destreaking" strategies for two-dimensional electrophoresis / F. Bai, S. Liu, and F.A. Witzmann -- Proteomic approaches to the study of rheumatoid arthritis / Mikaela Antonovici ... [et al.] -- Immunoglobulin patterns in health and disease / I. Miller and M. Goldfarb -- Difference gel electrophoresis (DIGEe) / M. Unlu and J. Minden -- Principles and challenges of basic protein separation by two-dimensional gel electrophoresis / Anton Posch, Aran Paulus, and Mary Grace Brubacher -- Multidimensional separation of membrane proteins / Susan Francis-McIntyre and Simon J. Gaskel -- Structural approaches to glycoproteomics / H. Geiser, Cristina Silvescu, V. Reinhold -- Enrichment and analysis of glycoproteins in the proteome / N. Packer, N. Karlsson, and N. Wilson -- Proteomic analyses using high efficiency separations and accurate mass measurements / Jon M. Jacobs and Richard D. Smith -- Middle-out proteomics / Scott J. Berger ... [et al.] -- Polymeric monolithic capillary columns in proteomics / Alexander R. Ivanov -- Staining proteins in polyacrylamide gels / Gary Smejkal -- Multiplexed proteomics / B. Schulenberg -- Direct immunoprobing on glyoxyl agarose composite gels / J.R. Shainoff -- NMR-driven chemical proteomics / P.K. Pullela and D.S. Sem -- Electrophoretic NMR / Qiuhong He and Xiangjin Song.
catalogue key
5817887
 
Includes bibliographical references and index.
A Look Inside
Reviews
This item was reviewed in:
SciTech Book News, March 2006
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Summaries
Long Description
Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. The editors take a broad survey of the field, avoiding bias to any particular method. Coverage extends across the entire process, from sample preparation to clinical and research applications such as drug and biomarker discovery. The book provides detailed discussions on prefractionation methods, electrophoresis, high performance liquid chromatography (HPLC), and NMR techniques, among others. Demonstrating applications to clinical and basic research, the text is ideal for scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.
Main Description
Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins.Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research.Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems.Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated "subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions.Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.
Main Description
Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions. Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.
Table of Contents
Sample Preparation
Applications of Pressure Cycling Technology (PCT) in Proteomicsp. 3
Applications of Ion-Exchange (IEX) Chromatography to Reduce Sample Complexity Prior to Two-Dimensional Gel Electrophoresis (2DE)p. 19
Separations in Proteomics: Use of Camelid Antibody Fragments in the Depletion and Enrichment of Human Plasma Proteins for Proteomics Applicationsp. 29
Novel Plasma Protein Separation Strategy Using Multiple Avian IgY Antibodies for Proteomic Analysisp. 41
Immunoaffinity Depletion of High-Abundant Proteins for Proteomic Sample Preparationp. 63
Isolation of Plasma Membrane Proteins for Proteomic Analysisp. 81
New Ultrafiltration and Solid Phase Extraction Techniques Improve Serum Peptide Detectionp. 89
Sample Prefractionation and Analysis
Tools for Sample Preparation and Prefractionation in Two-Dimensional (2D) Gel Electrophoresisp. 107
Optic Nerve Fractionation for Proteomicsp. 135
Fractionation of Retina for Proteomic Analysesp. 157
Reducing Protein Sample Complexity with Free Flow Electrophoresis (FFE)p. 187
Applications of Electrophoresis in Proteomics
Destreaking Strategies for Two-Dimensional Electrophoresisp. 207
Proteomic Approaches to the Study of Rheumatoid Arthritisp. 219
Immunoglobulin Patterns in Health and Diseasep. 235
Difference Gel Electrophoresis (DIGE)p. 269
Principles and Challenges of Basic Protein Separation by Two-Dimensional (2D) Electrophoresisp. 287
Multidimensional Separation of Membrane Proteinsp. 301
Structural Approaches in Glycoproteomicsp. 321
Enrichment and Analysis of Glycoproteins in the Proteomep. 345
Applications of High-Performance Liquid Chromatography
Proteomic Analyses Using High-Efficiency Separations and Accurate Mass Measurementsp. 363
Middle-Out Proteomics: Incorporating Multidimensional Protein Fractionation and Intact Protein Mass Analysis as Elements of a Proteomic Workflowp. 387
Polymeric Monolithic Capillary Columns in Proteomicsp. 419
Related Techniques
Proteins Staining in Polyacrylamide Gelsp. 439
Multiplexed Proteomics: Fluorescent Detection of Proteins, Glycoproteins, and Phosphoproteins in Two-Dimensional (2D) Gelsp. 453
Glyoxyl Agarose and Its Composite Gels: Advantageous Alternatives to Polyacrylamide Gel Electrophoresis for Very Large and Small Proteins and Peptides and All Sizes in Betweenp. 461
Nuclear Magnetic Resonance-Driven Chemical Proteomics: The Functional and Mechanistic Complement to Proteomicsp. 467
Electrophoretic Nuclear Magnetic Resonance in Proteomics: Toward High-Throughput Structural Characterization of Biological Signaling Processesp. 489
Indexp. 505
Table of Contents provided by Ingram. All Rights Reserved.

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