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In situ hybridization in neurobiology : advances in methodology /
edited by James H. Eberwine, Karen L. Valentino, Jack D. Barchas.
imprint
New York : Oxford University Press, 1994.
description
xiii, 199 p., [6] p. of plates : ill. (some col.) ; 24 cm.
ISBN
0195075072 (acid-free paper)
format(s)
Book
Holdings
More Details
imprint
New York : Oxford University Press, 1994.
isbn
0195075072 (acid-free paper)
catalogue key
422287
 
Includes bibliographical references and index.
A Look Inside
Reviews
Review Quotes
1. J. Eberwine: Methodological Considerations in the Utilization of In Situ Hybridization 2. C.D. Toran-Allerand and R.C. Miranda: Combining Non-Isotopic In Situ Hybridization Histochemistry with Steroid Autoradiography 3. P.C. Emson, B. Heppelman, and S.M. Augood: Development of Techniques to Combine Isotpic and Non-Isotopic In Situ Hybridization and Immunocytochemistry for Phenotypic Characterization of Individual Neurons 4. F. Baldino, Jr., E. Robbins, and M.E. Lewis: Enzyme Histochemical Detection of Neuronal mRNA 5. S.L. Petersen and S. McCrone: Characterization of the Receptor Complement of Individual Neurons Using Dual-Label In Situ Hybridization Histochemistry 6. P.M. Wise, et al.: Assessment of Peptide Secretion and Gene Expression within Individual Cells 7. O. Yeralan, et al.: Quantitative Micro-IIn Situ Hybridization 8. M.F. Chesselet and L.T. Weiss-Wunder: Quantification of In Situ Hybridization Histochemistry 9. J.L. Roberts and N. Levin: Analysis of Transcription Levels Using Intervening Sequence In Situ Hybridization 10. R.E. Tanzi and B.T. Hyman: Analysis of Alternatively-Transcribed Messages in Brain by In SItu Hybridization Using Biotinylated Oligonucleotide Probes 11. O. Bagasra, T. Seshamma, and R.J. Pomerantz: In Situ Polymerase Chain Reaction: A Powerful New Methodology 12. K. Miyashiro, et al.: In Situ Transcription: Methodology and Multi-Faceted Applications
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Summaries
Long Description
In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situ hybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridization technology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. It discusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.
Long Description
In situ hybridization has become an important and widely used research tool for neurobiologists. This new volume examines the methodological advances that have been made in in situ hybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs and both proteins and mRNAs within the same section, and methods for using in situ hybridization technology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.
Main Description
In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.
Table of Contents
Methodological Considerations in the Utilization of In Situ Hybridization
Combining Non-Isotopic In Situ Hybridization Histochemistry with Steroid Autoradiography
Development of Techniques to Combine Isotpic and Non-Isotopic In Situ Hybridization and Immunocytochemistry for Phenotypic Characterization of Individual Neurons
Enzyme Histochemical Detection of Neuronal mRNA
Characterization of the Receptor Complement of Individual Neurons Using Dual-Label In Situ Hybridization Histochemistry
Assessment of Peptide Secretion and Gene Expression within Individual Cells
Quantitative Micro-IIn Situ Hybridization
Quantification of In Situ Hybridization Histochemistry
Analysis of Transcription Levels Using Intervening Sequence In Situ Hybridization
Analysis of Alternatively-Transcribed Messages in Brain by In SItu Hybridization Using Biotinylated Oligonucleotide Probes
In Situ Polymerase Chain Reaction: A Powerful New Methodology
In Situ Transcription: Methodology and Multi-Faceted Applications
Table of Contents provided by Publisher. All Rights Reserved.

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