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Immune and receptor assays in theory and practice /
Patrick Englebienne.
Boca Raton : CRC Press, 2000.
392 p. : ill.
0849300614 (alk. paper)
More Details
Boca Raton : CRC Press, 2000.
0849300614 (alk. paper)
contents note
Ch. 1. Receptors at work -- Ch. 2. Receptors and antibodies -- Ch. 3. Receptor and antibody production -- Ch. 4. Molecular basis of ligand-receptor interaction -- Ch. 5. Immuno- and receptor-assay design -- Ch. 6. Analytical signal: generation and detection -- Ch. 7. The sample -- Ch. 8. Assay validation -- Ch. 9. Data analysis, processing, and presentation.
catalogue key
Includes bibliographical references and index.
A Look Inside
This item was reviewed in:
SciTech Book News, June 2000
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Main Description
In the past ten years, employment of immuno- and receptor- assays has grown dramatically. Now used in the pharmaceutical industry for automated screening programs, in the agro-food industry for on-line processing control and food adulteration detection, and in clinical laboratories, they are fully integrated analytical tools. However, the literature often covers only one type of assay or just one of the many systems available. Immune and Receptor Assays in Theory and Practice gathers and organizes the available information to help you establish the best assay for your application. This composite presents the fundamentals of both techniques and introduces practical examples of equation use, antibody and receptor purification, antigen labeling, immunization, and establishment of antibodies for long-term storage. It contrasts the many different assay designs and addresses market trends as the context for developing immuno-assay goals. In addition, this volume summarizes the biochemical and physical properties involved in antibody- and receptor-ligand interactions and reagent manufacture. This is the first, single-volume synthesis of both immuno- and receptor-assays. With theoretical background and practical examples, Immune and Receptor Assays in Theory and Practice allows you to base your experiment on proven techniques, components, and applications for the most reliable results.
Table of Contents
Introductionp. v
Receptors at Workp. 1
Bibliographical References and Further Readingp. 20
Receptors and Antibodiesp. 23
Origin and relatednessp. 23
Structure, biosynthesis, and interactionsp. 31
The steroid and thyroid hormone receptor superfamilyp. 32
The circulating steroid-binding proteinsp. 35
The immunoglobulin-like superfamilyp. 41
Biological, biosynthetic, and synthetic alternativesp. 48
Polyspecific antibodiesp. 49
Oligonucleotidesp. 50
Imprinted polymersp. 53
Bibliographical References and Further Readingp. 56
Receptor and Antibody Productionp. 59
Cell culture and receptor productionp. 59
Antigens, haptens, and immunogenicityp. 74
Production of monoclonal and polyclonal antibodiesp. 90
Extraction, purification, and stabilization of receptors and antibodiesp. 105
Bibliographical References and Further Readingp. 135
Molecular Basis of Ligand-Receptor Interactionp. 141
The molecular recognition processp. 141
Kinetics of ligand-receptor interactionsp. 145
Physical aspects of ligand-receptor interactionsp. 158
Factors influencing ligand-receptor interactionsp. 171
Bibliographical References and Further Readingp. 178
Immuno- and Receptor-Assay Designp. 181
Competitive versus noncompetitive assayp. 181
Homogeneous versus heterogeneous--liquid versus solid phase assayp. 189
Agglutination and precipitation assaysp. 200
Assays using labeled reagentsp. 212
Market or technology driven assay designp. 222
Bibliographical References and Further Readingp. 223
Analytical Signal: Generation and Detectionp. 227
Labels and their measurementp. 227
Techniques of labelingp. 253
Tracer purification and stabilizationp. 266
Biosensorsp. 274
Instrumentationp. 277
Bibliographical References and Further Readingp. 285
The Samplep. 291
Insoluble versus soluble analytep. 291
Matrix effectsp. 300
Standardizationp. 311
Bibliographical References and Further Readingp. 322
Assay Validationp. 325
The reference methodp. 325
Specificity and sensitivityp. 338
Interferencesp. 344
Bibliographical References and Further Readingp. 352
Data Analysis, Processing, and Presentationp. 355
The dose-response curvep. 355
Binding datap. 362
Reagent stability follow-upp. 372
Assay validationp. 377
Bibliographical References and Further Readingp. 380
Indexp. 381
Table of Contents provided by Syndetics. All Rights Reserved.

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