Catalogue


DNA topoisomerase protocols /
edited by Mary-Ann Bjornsti and Neil Osheroff.
imprint
Totowa, N.J. : Humana Press, c1999-c2001.
description
2 v. : ill.
ISBN
0896034445 (v. 1 : alk. paper) 0896035123 (v. 2)
format(s)
Book
Holdings
More Details
imprint
Totowa, N.J. : Humana Press, c1999-c2001.
isbn
0896034445 (v. 1 : alk. paper) 0896035123 (v. 2)
contents note
v. 1. DNA topology and enzymes -- v. 2. Enzymology and drugs.
catalogue key
2978560
 
Includes bibliographical references and index.
A Look Inside
Summaries
Back Cover Copy
DNA Topoisomerase Protocols, I: DNA Topology and Enzymes brings together an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, and bacterial, yeast and mammalian expression systems. A companion volume, DNA Topoisomerase Protocols, v. 2: Enzymology and Drugs, provides detailed protocols to study the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs.DNA Topoisomerase Protocols, I: DNA Topology and Enzymes will be of immense value to the many basic scientists and clinicians who want better to understand and to exploit proficiently the wealth of recent discoveries about chromatin structure and its relation to gene expression, about DNA topoisomerases as the targets of antitumor and antibacterial agents, and about DNA repair.
Main Description
DNA Topoisomerase Protocols is an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these time-proven methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, bacterial, yeast, and mammalian expression systems.
Table of Contents
Prefacep. v
Contents of Companion Volumep. xi
Contributorsp. xiii
Introduction to DNA Topoisomerasesp. 1
Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresisp. 9
Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomersp. 19
Analysis of Altered DNA Structures: Cruciform DNAp. 29
Purification of Supercoiled Plasmid DNAp. 41
Purification and Use of DNA Minicircles with Different Linking Numbersp. 51
Isolation of Kinetoplast DNAp. 61
Isolation of Knotted DNA from Coliphage P4p. 69
Analysis of DNA Knots and Catenanes by Agarose-Gel Electrophoresisp. 75
Sedimentation Analysis of Bacterial Nucleoid Structurep. 87
Coating DNA with RecA Protein to Distinguish DNA Path by Electron Microscopyp. 99
Methods for Analyzing DNA Bendingp. 109
Formation of Extrachromosomal DNA Rings in Saccharomyces cerevisiae Using Site-Specific Recombinationp. 125
Overexpression and Purification of Bacterial DNA Gyrasep. 135
Overexpression and Purification of Bacterial DNA Topoisomerase Ip. 145
Overexpression and Purification of Escherichia coli DNA Topoisomerase IIIp. 153
Overexpression and Purification of Bacterial Topoisomerase IVp. 163
Purification of the Bacteriophage T4 Type II DNA Topoisomerasep. 171
Overexpression and Purification of DNA Topoisomerase I from Yeastp. 179
Overexpression and Purification of Saccharomyces cerevisiae DNA Topoisomerase II from Yeastp. 187
Purification of DNA Topoisomerase II from Drosophila melanogasterp. 199
Purification of DNA Topoisomerase I from Human Placentap. 213
Purification of Baculovirus-Expressed Human DNA Topoisomerase Ip. 223
Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatographyp. 235
Metabolic Labeling, Immunoprecipitation, and Two-Dimensional Tryptic Phosphopeptide Mapping of Human Topoisomerase IIp. 243
Immunoblot Analysis and Band Depletion Assaysp. 253
Visualization of DNA Topoisomerases by Electron Microscopyp. 269
Compendium of DNA Topoisomerase Sequencesp. 279
Indexp. 317
Table of Contents provided by Syndetics. All Rights Reserved.

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