Neutrophil methods and protocols [electronic resource] /
edited by Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch.
imprint
Totowa, N.J. : Humana Press, c2007.
description
xx, 537 p. : ill. (some col.) ; 24 cm.
ISBN
1588297888 (alk. paper), 9781588297884 (alk. paper)
format(s)
Book
More Details
imprint
Totowa, N.J. : Humana Press, c2007.
isbn
1588297888 (alk. paper)
9781588297884 (alk. paper)
restrictions
Licensed for access by U. of T. users.
catalogue key
8006983
 
Includes bibliographical references and index.
A Look Inside
Reviews
Review Quotes
From the reviews:"...a carefully compiled collection of protocols that allow for study of many aspects of the neutrophil." -Doody's Book Review, Weighted Numerical Score:85-3 Stars"This excellent book is one of the latest in the 'Methods in Molecular Biology' series … . it comes beautifully presented and bound in a hardback cover and would grace any bookshelf. I would urge all self-respecting laboratories working with neutrophils whether in research or diagnostics to purchase one immediately. … I was impressed by this book's comprehensive provision of protocols, addressing all aspects of neutrophil function and physiology, and including an excellent section on diagnosis of both inherited and non-inherited neutrophil disorders." (Lynn Morgan, Immunology News, May, 2008)
This item was reviewed in:
Doody's Reviews, October 2007
SciTech Book News, December 2007
To find out how to look for other reviews, please see our guides to finding book reviews in the Sciences or Social Sciences and Humanities.
Summaries
Bowker Data Service Summary
'Neutrophil Methods and Protocols' provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders.
Main Description
Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis, phagocytosis and bacterial activity. Part 4 presents protocols measuring gene expression in neutrophils, and Part 5 concludes with assays for diagnosing the most common neutrophil disorders. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.
Main Description
This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.
Table of Contents
Neutrophils
Methods and Protocols
Edited
Contents
Dedicationp. i
Prefacep. ii
Contributorsp. v
Neutrophils: An Overview
The Role of Neutrophils in the Immune System: An Overview
Neutrophil Isolation And Subcellular Fractionation
Isolation of Human Neutrophils From Venous Blood
Neutrophil Isolation From Non-Human Species
Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
Biochemistry, Biology And Signal Transduction Of Neutrophils
Rac and Rap GTPase Activation Assays
Measurement of Phospholipid Metabolism in Intact Neutrophils
Analysis of Protein Phosphorylation in Human Neutrophils
Mitogen-Activated Protein Kinase Assays
Transduction of Proteins into Intact Neutrophils
Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils
Analysis of Electrophysiological Properties and Responses of Neutrophils
Analysis of Neutrophil Apoptosis
Neutrophil Adhesion And Chemotaxis
Optical and Fluorescence Detection of Neutrophil Integrin Activation
Analysis of Neutrophil Polarization and Chemotaxis
Quantifying and Localizing Actin Free Barbed Ends in Neutrophils
Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow
Model Systems to Investigate Neutrophil Adhesion and Chemotaxis
Neutrophil Phagocytosis And Bactericidal Activity
Immunofluorescence and Confocal Microscopy of Neutrophils
Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy
Analysis of Neutrophil Membrane Traffic During Phagocytosis
Analysis of Neutrophil Bactericidal Activity
A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions
Nadph Oxidase And Production Of Reactive Oxygen Species
Measurement of Respiratory Burst Products Generated by Professional Phagocytes
The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function
Cell-free Assays: The Reductionist Approach to the Study of NADPH Oxidase Assembly
Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics
Analysis Of Neutrophil Gene Expression And Transcription Factors
Genome-scale Transcript Analyses with Human Neutrophils
Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR
Detection of Intact Transcription Factors in Human Neutrophils
Neutrophil Defects And Diagnosis
Disorders of Neutrophil Function: An Overview
Table of Contents provided by Publisher. All Rights Reserved.

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